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Web Partner
Lab-on-a-Chip.comSponsoring Publications:
Asia Pacific Biotech
Micromachine Devices
The ScientistThese tools have essentially become the workhorses of the modern wet lab. Great efforts have been made to fully integrate them within themselves and within drug discovery pipelines. The results have been mixed. The numerous options available, from printed to spotted arrays, on a wide variety of surfaces and with different modes of quantification, place a premium on insuring validation of data, comparisons, and ways to improve interpretation of the large data sets of resulting data. Discussions will focus on those applications that can take greatest advantage and improve the performance of these devices. We should also note that Asia's impressive history in quality miniaturized manufacturing should play an increasingly important role in their production.
KEYNOTE SPEAKER
Dr. Charles Cantor, Sequenom Inc.SESSION CHAIRS
Dr. Yoshinobu Baba, University of Tokushima
Dr. Ian Humphery-Smith, Glaucus Proteomics B.V.
Dr. Sebastian Meier-Ewert, GPC Biotech
Dr. Shigeori Takenaka, Kyushu UniversityADDITIONAL SPEAKERS
Mr. Akihiro Arai, Shimadzu Corporation
Dr. Gianfranco de Feo, Affymetrix Inc.
Dr. Paz Einat, Quark Biotech (U.S.) and QBI Enterprises
Dr. Stephen Friend, Rosetta Inpharmatics, Inc.
Dr. Harold (Skip) Garner, University of Texas Southwestern Medical Center
Dr. James Gilmore, Genometrix, Inc.
Dr. Hisashi Hagiwara, Hitachi Electronics Engineering Co, Ltd.
Dr. Martin Hoffman, LION bioscience AG
Dr. Christopher Hopkins, Incyte Genomics Inc.
Dr. Eiichiro Ichiishi, Kyoto Prefectural University of Medicine
Dr. Takehiko Kitamori, The University of Tokyo
Dr. Kazuhiko Nakatani, Kyoto University
Dr. Steffen Nock, Zyomyx, Inc.
Dr. Bill Pagels, BioMicro Systems, Inc.
Dr. Christopher Pohl, Ciphergen Biosystems, Inc.
Dr. Gozoh Tsujimoto, National Children's Medical Research Center
Dr. Henk van Damme, PamGene B.V.
Dr. Francois Vinet, LETI, CEA-Grenoble
Dr. Youmin Wang, Medical College of Wisconsin
Dr. Alon Wassermann, Compugen
Dr. Bernhard Weigl, Micronics, Inc.
MANUFACTURING/DESIGN
Advantages of the GeneChip Approach
Customization of Arrays
Capillary Array Electrophoresis
Improved Biochip Sensitivity
Design Using the Transcriptome
Electrophoretic Device
Real-Time Reaction MonitoringDETECTION AND ANALYSIS
Spectral Analysis of One Dalton Sensitivity
Ferrocenyl Naphthalene Diimide as a Hybridization Indicator
Fluorescence Intensity Model
Designing and Synthesizing Ligands for the G-G Mismatch
The Entire Genome as a Sensor Pad
DataBase Collection and AnnotationLAB-ON-A-CHIP/ PROTEIN CHIPS
Simple, Stable, Microfluidic Technology
Diffusion-Based Separation and Detection
How PCR Fits In
Integration Emplying Photothermal Single Molecule Detection
High-Throughput Micro-ELISA Arrays
Integration of Advanced Materials, Protein Engineering, and Detection Physics
High-throughput and Reproducible Analyses of the Human ProteomeAPPLICATIONS
Comparative Study of DNA Chips with Cell Signaling Network Data Base
Comprehensive Assignment of Biological Function
G-Protein Coupled Receptors Involved in Disease States
Associating Drug or Compound Action with a Measurable Biological Readout
T-Cell Activation Analysis
SUNDAY, JUNE 3
4:00-6:00pm Early Registration and Poster and Exhibit Setup
MONDAY, JUNE 4
7:30am Registration, Poster and Exhibit Viewing, and Light Continental Breakfast
MANUFACTURING/DESIGN
8:30 Chair's Opening Remarks
Dr. Yoshinobu Baba, Professor of Physical Chemistry and Project Director of CREST, Japan Science and Technology Corporation; Project Director of NEDO, MITI, University of Tokushima8:35 The Use of GeneChip Technology for Gene expression and Genotyping Applications
Dr. Gianfranco de Feo, Program Manager, Genomic Collaborations, Affymetrix Inc.
GeneChip Technology has been recognized as a platform of choice for many academic and pharmaceutical researchers. I will briefly discuss some of the unique features of the technology, and the advantages they bring to highly parallel gene expression and genotyping studies. I will then review several key studies that were performed by researchers using the technology.9:05 Digital Optical Chemistry: Custom Oligonucleotide Microarrays Using UV Light Projection
Dr. Harold (Skip) Garner, University of Texas Southwestern Medical Center
The heart of the system is a device based on the Texas Instruments Digital Light Processor, a chip with hundreds of thousands to millions of mirrors under computer digital control. This system was integrated into a device that also contains a sample holder and a computer controlled reagent delivery system based on oligo synthesis technology from Affymetrix and our own Mermade system. The oligo arrays can be designed from known DNA sequence using the UTSW code, PRIMO.9:35 Micro- and Nanofabricated Chip Technology for Single DNA Molecule Analysis and Genomic Polymorphism Analysis
Dr. Yoshinobu Baba
Capillary array electrophoresis plays a vital role in the genome sequencing era, but in the post human genome sequencing era further development of analytical technology for DNA, mRNA, protein, and metabolites is highly required. We have been challenged to develop the novel technology for ultrafast DNA analysis by the microfabricated chip-based integration of all processes required for human genome analysis. I will describe our development for field-inversion electrophoresis on a chip for high-resolution separation of DNA, SSCP analysis on a chip for SNP analysis, a novel method for stretching the single DNA molecule on a chip for single molecule mapping, and novel technology for manipulation of single DNA molecule on a chip for single genomic DNA analysis. The possibility of development of nanochip technology will be discussed. I will also discuss the application of microscale technology to proteomics, including fast separation of proteins and peptides, cell-free amplification of proteins, and integrated microchip for protein analysis.10:05 Poster and Exhibit Viewing, Refreshment Break
10:45 Oligonucleotides Biochips for Highly Sensitive Biological Tools
Dr. Francois Vinet, Senior Researcher, LETI, CEA-Grenoble
Sensitivity and reproducibility are the major drawbacks of the biochips technology today. In such a background, we will compare the performances of both in situ synthesis and covalent immobilization of oligonucleotides chips we have developed. Moreover, a new technology for the improvement of biochip sensitivity will be presented as well as the biological validation with samples of low expression genes.11:15 DNA Chip Design Using the Transcriptome: A Novel Approach to Improve Sensitivity
Dr. Alon Wassermann, Head, Chip Design Group, Compugen
The design of DNA chips requires careful balancing of biological and chemical features. Specifically, there is a need to select probes that best represent the collection of genes targeted by the assay. Where multiple probes are selected per gene, the Exxon distribution of such probes becomes a central issue. Alternative splicing and relative expected expression levels must be taken into account when choosing and designing the probes. Compugen's LEADS technology, combined with information from Enzyme's SAGE libraries and molecular biology assays, has been successfully applied in optimizing such oligo-based chips. Results of such experiments will be described in the talk, and an intuitive, webbed interface to access chip data will be demonstrated.11:45 Development of a High-Speed Electrophoretic Device
Dr. Hisashi Hagiwara, Principal Engineer, Bio-system Equipment Design, Hitachi Electronics Engineering Co., Ltd.
We have developed a high-speed electrophoretic device using microfabrication technology. This device is cheaply assembled since a semiconductor element was used in the optical system (illuminant and detector) and the device is totally miniaturized. We have started its sales in JAPAN in September 2000 at a price of 1,500,000. The device consists of a disposable channel-contained plastic chip, reagents including polymer matrix and size standards, and a EtBr fluorescence detector. The electrophoresis device is computer-controlled and the result could be automatically analyzed by a provided software. The available chip is plastic-made (PMMA:polymethyl metacrylate: 85 mm × 50 mm; Hitachi Chemical Co., Ltd. made a chip and reagent kit). A separation of a DNA size-marker and a PCR product were finished in four minutes.
[This works was performed as a part of the research and development project of the industrial science and technology program supported by NEDO (New Energy and Industrial Technology Development Organization).]12:15 An Enhanced Microarray Format for Post-genomic Applications
Dr. Henk van Damme, Senior Consultant, PamGene B.V.
A microarray platform with flow-through dynamic properties that allows real-time monitoring of the reaction will be described. Hybridization can be observed within ten minutes and the signal kinetics is complete after thirty minutes. Data on the kinetics of incubation will be presented in terms of temperature effects, probe-target concentration curves, and time course studies, as well as work performed in conjunction with our Japanese industrial collaborator.12:45 Lunch (on your own)
DETECTION AND ANALYSIS
2:00 Chair's Remarks
Dr. Shigeori Takenaka, Associate Professor, Kyushu University2:05 Keynote Presentation
Analyzing DNA Chips by Automated Mass Spectrometry
Dr. Charles Cantor, Sequenom Inc.2:45 A Multi-electrode DNA Sensing System of the Next Generation
Dr. Shigeori Takenaka
DNA chips and DNA microarrays are one of the most promising techniques to enable high-throughput analysis of a huge spectrum of genes or expressed mRNA in an organism. We have been developing an electrochemical DNA sensing system based on ferrocenyl naphthalene diimide as a specific hybrid indicator coupled with a DNA probe immobilized on the electrode. This technique may be extended to the multi-electrode array system having a potential DNA chip of the next generation.3:15 Refined Mismatch Detecting Sensor
Dr. Kazuhiko Nakatani, Associate Professor, Faculty of Engineering, Kyoto University
To develop a conceptually new method for rapid discovery and detection of single nucleotide polymorphisms (SNPs), we have succeeded for the first time in designing and synthesizing ligands that specifically bind with high affinity (Kd = 53 nM) to the guanine (G)-guanine mismatch, one of four SNP types. Detection of the G-G mismatch was performed by a surface plasmon resonance (SPR) assay using a sensor chip carrying the G-G specific ligand on its surface. This assay was found applicable for the detection of SNP existing in PCR amplification products of 652 nucleotides sequence of the HSP70-2 gene.3:45 Poster and Exhibit Viewing, Refreshment Break
4:30 Modeling and Experimentation of Probe and Target Concentrations on Fluorescence Intensity of cDNA Microarray
Dr. Youmin Wang, Medical College of Wisconsin
To investigate the effect of probe and target concentrations on quantifying differential gene expression in microarray analysis, a theoretical model was developed and tested. A series of known genes with varying concentrations were spotted onto the glass slides and hybridized with target RNAs. The theoretical model fits remarkably well with the experimental data and will be useful for quantifying target material.5:00 Genomic Sensor Pads and Pattern Recognition: Impacting Both the Discovery and Development of Drugs
Dr. Stephen Friend, President and Chief Executive Officer, Rosetta Inpharmatics, Inc.
Neither transcript levels nor protein levels directly define the functions of genes or the activities of compounds. By using the entire genome as a sensor pad either at a transcript level or at a protein level, it is possible using pattern matching to interpret unknown compounds and genes in terms of known references if coherent data sets are used. The focus will be on specific solutions to actual unmet needs in target identification, lead compound optimization, and safety and efficacy determininations required to shorten the overall drug discovery pipeline.5:30 An Integrated Expression Profiling Set-Up for a Drug Discovery Process
Dr. Martin Hofmann, Senior Scientist. LION bioscience AG
Abstract unavailable at time of printing6:00-7:30 Reception in Exhibit Hall (sponsored by Cambridge Healthtech Institute)
TUESDAY, JUNE 5
7:30am Poster and Exhibit Viewing and Light Continental Breakfast
LAB-ON-A-CHIP AND PROTEIN CHIPS
8:00 Chair's Remarks
Dr. Ian Humphery-Smith, Chief Operating Officer, Glaucus Proteomics B.V.8:05 Sample Processing and Analysis Using Novel Microfluidic Technologies
Dr. Bill Pagels, BioMicro Systems, Inc.
Microfluidic technologies have been employed in genomics, diagnostics, and drug development to manipulate and analyze samples in methodologies of varying complexity. We have developed Passive Fluid Control™ microfluidics to rapidly fabricate inexpensive biochips capable of massively parallel sample processing and interfacing with existing equipment systems. This simple, stable microfluidic technology requires no sensors, biochip electronics, or moving parts. Applications of this unique microfluidic technology in the above areas will be discussed.8:35 Lab-on-a-Chip Plastic Disposable Technology: Applications of Microfluidic Laminar Flow Diffusion Structures in Drug Development
Dr. Bernhard Weigl, Manager, Business Development, and Senior Scientist, Micronics, Inc.
Micronics' proprietary diffusion-based separation and detection structures (H-Filters™ and T-Sensors™) are currently in development in a number of projects for upstream sample processing for HTS and PCR processes. New exciting applications of this technology are in lead compound screening and in cell toxicity monitoring.9:05 PCR Product Sizing in Microchip Electrophoresis System Utilizing a Novel Linear Imaging UV Detector
Mr. Akihiro Arai, LC R&D, Analytical Instruments Div, Shimadzu Corporation
Microfabricated or microchip electrophoresis has achieved remarkably rapid development especially in DNA separation arena, and instrumentation continues to advance toward a fully automated tool. The Shimadzu MCE 2010 provides 96-well PCR reaction tube compatible auto sampler, and novel linear imaging UV detector in which the entire region of the separation channel is monitored using a 1024-element photodiode. The multiple data acquired from each diode can be averaged together over certain period of time during eliminating the potential for separation to improve the signal-to-noise ratio. The specially designed quartz chip and the analytical conditions are optimized to give the best separation of DNA fragments and also some example of PCR products will be demonstrated.9:35 Chemical Proscessing in Microchanel Network on Chip
Dr. Takehiko Kitamori, Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo. Integrated Chemistry Project, Kanagawa Academy of Science and Technology
Unit operations of chemical processing such as mixing, reaction, separation, phase separation in continuous microflow network were developed by using spontaneous behavior of molecules in liquid. Design and fabrication techniques were also established for any kind of chemical system. The examples of integration of wet analysis and organic synthesis systems will be introduced in this lecture.10:05 Poster and Exhibit Viewing, Refreshment Break
10:45 Cytokine Protein Profiling: Use of High-Throughput Micro-ELISA Arrays
Dr. James Gilmore, Director of Product Development, Genometrix, Inc.
The ability to screen and analyze thousands of samples across a hypothesis-driven set of antibodies or antigens may be a valuable tool to advance proteomic research. Genometrix has developed a microarray platform that simultaneously quantitates up to 25 analytes while utilizing 25ul of sample. Dynamic range is approximately 400,000-fold (2 mg/ml to 4 pg/ml). Discussion will focus on comparison of microarray-derived PSA values to ELISA-derived PSA values, disease cytokine profiling, and software analysis tools.11:15 Protein Microarrays as New Tools in Proteomics
Dr. Steffen Nock, Sr. Director of Biochemistry, ZYOMYX,Inc.
Novel high-throughput biological applications in the drug discovery process, disease diagnosis, and the development and application of patient-specific medicines require highly parallel, miniaturized device technology applied to proteins and their biochemical pathways. While technological innovation has adapted the analysis of genetic material to a miniaturized format, the more delicate nature of protein structures has precluded the development of analogous devices for proteins. Protein microarrays have started to emerge recently based on new developments and integration efforts in advanced materials, protein engineering, and detection physics. Recent developments and selected examples will be presented with an emphasis on the technical challenges in surface and assay methodologies.11:45 Peptide, protein and antibody arrays as an integrated approach to following human tissues in health and disease
Dr. Ian Humphery-Smith
Glaucus Proteomics has developed a molecularly-sound and fully-scalable approach to the task of undertaking meaningful high-throughput and reproducible analyses of the Human Proteome in health and disease. This will facilitate large sample cohorts accompanied by high levels of proteomic coverage beyond that possible by traditional technologies. Analysis of the simplest living organisms (Mycoplasmas) over the last decade and currently the publication of the most complete proteome have taught us that 'traditional proteomics' (2D gels, advanced image analysis and mass spectrometry) is totally inadequate for a major push on the Human Proteome. Thus, our integrated approach links parallel generation and screening of antibodies, molecular expression vectors for immobilization and immunization, extreme high-throughput screening engines, 'genome-wide' triple protein enrichment including 'on-array' final purification, proprietary surface chemistries critical to effective HTS Western Blotting, and peptide-based 'proteomic closure' strategies to allow screening of the 'near-to total' proteome in living tissues. Complementary biocomputing activities are focused on the development of dynamic data extraction tools designed to highlight biologically-relevant permutations and combinations from within multi-vector datasets.12:15 Recent Advances in ProteinChip Technology for the Analysis of Proteins
Dr. Christopher Pohl, Vice President of R&D, Ciphergen Biosystems, Inc.
Ciphergen's ProteinChip technology utilizes the proprietary Surface Enhanced Laser Desorption/Ionization (SELDI) to integrate the functions of protein capture, protein purification and protein characterization. Initial technology has focused upon two classes of materials: "chromatographic" surfaces that enable selective retention of proteins based on standard chromatographic principles as well as activated surfaces which allow covalent coupling of proteins directly to chip surfaces. Chromatographic surfaces have proven to be extremely useful in the discovery mode for profiling of biological media, whereas activated surfaces have found use in a wide variety of protein interaction studies. The combination of these selective surfaces with SELDI-TOF-mass spectrometry allows the production of arrays of very high information density.
This presentation will focus on recent advances in ProteinChip array chemistry in both of the above areas. These advances will be demonstrated with specific illustrative examples that demonstrate the performance improvements of these new advanced surfaces and their application to a wide variety of real biological systems.12:45 Lunch in Exhibit Hall (sponsored by Cambridge Healthtech Institute)
Applications
2:00 Chair's Remarks
Dr. Sebastian Meier-Ewert, Vice President and Head of Research, GPC Biotech2:05 Identification of the Genes Responsible to Anticancer Promoting Effect: Application of the Combination of DNA Chip Technology and Cell Signaling Network Data Base
Dr. Eiichiro Ichiishi, Kyoto Prefectural University of Medicine
In this study, we considered if DNA chip technology is necessary to further clarify the long-term issues. As one trial, we compared a large amount of data in DNA chips with Cell Signaling Network Data Base (CSNDB) maintained by the National Institute of Health Sciences and investigated its roles in intracellular signal transmission.2:35 The BiFAR™ Platform: High-Throughput Identification of Key Genes Involved in Disease-Related Cellular Response
Dr. Paz Einat, Chief Scientist, Quark Biotech (U.S.) and QBI Enterprises
QBI's BiFAR™ (Biological Integrated Functional Array) platform enables a comprehensive assignment of biological function to all expressed genes, the human transcriptosome, in virtually any disease context. The technology uses a high-throughput, high-fidelity process to identify the positive and negative modulators of a studied phenotype. It includes a seamless integration with disease-related expression profiling and provides a direct approach for assigning function to disease-associated genes.3:05 Functional Genomic Search Using Microarrays with Normalized cDNA Library
Dr. Gozoh Tsujimoto, National Children's Medical Research Center
Large-scale monitoring of gene expression is a powerful approach to clarify the cellular events. DNA microarray technologies permit us to recognize genomewide expression profiling and bring a profound impact to biological research such as drug discovery and molecular classification of diseases. We introduced microarrays to discover novel function of G-protein coupled receptors (GPCRs) involved in disease states. I will present our recent works on microarray analysis of disease model animals with normalized cDNA library.3:35 Poster and Exhibit Viewing, Refreshment Break
4:00 Genomic Tools for Mode of Action Analysis in Drug Discovery
Dr. Sebastian Meier-Ewert
Recently, the use of gene expression analysis has emerged as a powerful approach to associating drug or compound action with a measurable biological readout. The approach is to measure complex patterns of gene expression changes that are highly specific to the exact molecular action of a compound. Such complex expression patterns can be used for drug discovery in a number of ways including the assessment of drug specificity and mode of action analyses. In order to perform such analyses it has been necessary to (1) set up a robust workflow process with capacity of hybridization up to 100 arrays, each containing up to 13,000 genes, per day; (2) introduce visualization tools for QC of data; and (3) implement data analysis algorithms for pattern recognition and gene-gene correlations. These developments will be presented together with data from a number of evaluations of this approach in both whole bacterial ORFs (H. pylori and B. subtilis) and mammalian cDNA (human, mouse, and rat) systems.4:30 Microarray Analysis of mRNA Levels during T-Cell Activation
Dr. Christopher Hopkins, Senior Scientist, Incyte Genomics Inc.
Optimal T-cell activation requires two distinct stimuli initiated by cell surface interactions of the T cell with an antigen presenting cell (APC)-the signal transmitted through the T cell receptor-CD3 complex and the "co-stimulatory" signal associated with CD28. Jurkat, a leukemia T-cell line that grows in vitro without stimulation, was treated with mouse antibodies against human CD3 and human CD28. This treatment is known to simulate the activation process that is normally caused through the interaction with APC. PolyA RNA was prepared from cells treated for 0, 0.5, 1, 2, 4, and 8 hrs. Each time point was compared to a nontreated control in microarray hybridizations that screened over 700,000 elements representing over 15,800 gene clusters for differential expression. Differentially expressed genes were analyzed for their temporal expression patterns and possible roles in the two signal pathways. Over 60 genes of known, and 32 of unknown, functions were found to be differentially expressed by our criteria. Several of the identified genes are attractive drug target candidates. A subclass of the differentially expressed genes was analyzed for their potential involvement in the two different signals. Several genes were shown to be more associated with either the single or dual treatments.5:00 Close of Conference
HOTEL INFORMATION
Keio Plaza Inter-Continental Tokyo
2-2-1, Nishi-Shinjuku
Shinjuku-ku
Tokyo, Japan 160-8330
T: 81-3344-0111 o F: 81-3-3345-8269
Room Rates: Y16,000/S o Y18,000/D
To Reserve a Room:
You must call the hotel's Los Angeles office at 800-222-5346 or 213-362-7767 or fax 213-362-7772, attn: Ms. Mina Segal.
Please identify yourself as a Cambridge Healthtech Institute attendee to receive this special discounted rate.TRAVEL INFORMATION
Special Airline Discounts Available
Special discount fares have been established on United Airlines for this conference for flights originating in the US only. Please call Great International and National Travel at 617-527-0800 and ask for Joyce Dunn or e-mail her at jdunn@greatintltravel.com. Or you may call the United Airlines International Reservations Center directly at 800-521-4041. You must reference ID # 550WT.CALL FOR EXHIBITORS
Delegates at Microarrays and Microchips Japan will comprise the top managers and lab directors involved with clinical research at both commercial and academic institutes. Companies with services or products relating to lab automation and the collection, handling, storage, and final microarray analysis of nucleic acid samples, or those who want to enhance their exposure in the Japanese market, should seriously consider exhibiting at this event. Please contact John Rodolewicz at 617-630-1352 to obtain an exhibitor package. Exhibit space is limited so call now and secure this opportunity for your company.CALL FOR POSTERS
Cambridge Healthtech Institute encourages attendees to gain further exposure by presenting their work in the poster sessions. Please fill out the registration form, with the poster title and primary author. To ensure inclusion in the conference binder, a one-page summary must be submitted and registration must be paid in full by April 30, 2001.
POSTER INSTRUCTIONS
Phone: 617-630-1300
Fax: 617-630-1325
Email: chi@healthtech.com